Engineering and development of model lipid membranes mimicking the HeLa cell membrane

Cells are complex systems whose interaction with nanocarriers, i.e., liposomes, continuously under investigation to improve drug uptake. Model membranes can facilitate the understanding of processes involved in fusion or endocytosis. In this work, we engineered two different lipid model membranes, vesicles and supported bilayers (SLBs), mimicking composition HeLa cell plasma membrane. We characterized using atomic force microscopy (AFM) fluorescence. found that liposomes formed four components bilayer show a liquid ordered fluid nature between 13 °C 34 yield featureless SLBs onto mica. evaluated positively charged without cholesterol by AFM-based spectroscopy fluorescence techniques, such as Förster resonance energy transfer, lifetime decay anisotropy. The results indicated primary electrostatic liposomes. It was also confirmed well-known fact enhances process bilayer. All support usefulness rationale design for delivery. membrane POPC, POPE, POPS cholesterol. studied POPC:DOTAP membrane: i) flat characterised AFM after formation bilayers, b) vesicle FRET • A is characterised. HeLa-mimicking phase coexistence at room temperature. Laterally segregated domains not observed Supported Lipid Bilayers. Fusion changes Young’s modulus bilayers. POPC:CHOL:DOTAP fusing HeLa- Changes microviscosity corroborate process.